LeHavreV1, France, Analysis, bibRecord, 000C29

Mass distribution, polydispersity and focusing properties of carrier ampholytes for IEF II: pH 4–6 intervals

Identifieur interne : 000C29 ( France/Analysis ); précédent : 000C28; suivant : 000C30

Mass distribution, polydispersity and focusing properties of carrier ampholytes for IEF II: pH 4–6 intervals

Auteurs : Carolina Sim [Italie] ; Martha Elena Mendieta [Italie] ; Paolo Antonioli [Italie] ; Roberto Sebastiano [Italie] ; Attilio Citterio [Italie] ; Alejandro Cifuentes [Espagne] ; Gabriel Peltre [France] ; Pier Giorgio Righetti [Italie]

Source :

ELECTROPHORESIS [ 0173-0835 ] ; 2006-12.

RBID : ISTEX:D95CC745D7F7AD572C00973EEF3CA7E62040D88F

English descriptors

KwdEn :
- CE, Carrier ampholytes, IEF, MS.

Abstract

For studying the Mr distribution and number of species in narrow‐range (2 pH‐unit wide, in the nominal pI 4–6 interval) carrier ampholytes from four commercial sources (Bio‐Lyte, Servalyt, Ampholine and Pharmalyte), a 2‐D technique was adopted consisting of a focusing step in a liquid phase (Rotofor, yielding 20 fractions) followed by orthogonal CE in both, acidic and basic buffers. As a final step, every other fraction was analyzed by CE‐MS. The findings: Ampholine contains 80 different Mr compounds, in the Mr interval 203 to 893 Da, for a total of 325 isoforms. Bio‐Lyte consists of 66 different Mr species, in the Mr range 388 to 835 Da, for a total of 436 isoforms. Servalyt is made of 199 different Mr compounds, in the Mr interval 204 to 907 Da, for a total of 1302 isoforms. Pharmalyte pH 4–6.5, comprises 217 amphoteres, in the Mr range 150 to 1179 Da, for a total of 812 isoforms. Pharmalyte appears to be the best brand, with the vast majority of species focusing sharply at their pI position and <5% “poor” species, distributed along the entire pH gradient, denoting an extremely shallow pH/mobility curve across the pI value.

Url:

https://api.istex.fr/document/D95CC745D7F7AD572C00973EEF3CA7E62040D88F/fulltext/pdf

DOI: 10.1002/elps.200600344

Affiliations:

Links toward previous steps (curation, corpus...)

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to stream Main, to step Curation: 001107
to stream Main, to step Exploration: 001107
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ISTEX:D95CC745D7F7AD572C00973EEF3CA7E62040D88F

Le document en format XML

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<author><name sortKey="Sim, Carolina" sort="Sim, Carolina" uniqKey="Sim C" first="Carolina" last="Sim">Carolina Sim</name>
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<author><name sortKey="Mendieta, Martha Elena" sort="Mendieta, Martha Elena" uniqKey="Mendieta M" first="Martha Elena" last="Mendieta">Martha Elena Mendieta</name>
</author>
<author><name sortKey="Antonioli, Paolo" sort="Antonioli, Paolo" uniqKey="Antonioli P" first="Paolo" last="Antonioli">Paolo Antonioli</name>
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<author><name sortKey="Sebastiano, Roberto" sort="Sebastiano, Roberto" uniqKey="Sebastiano R" first="Roberto" last="Sebastiano">Roberto Sebastiano</name>
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<author><name sortKey="Citterio, Attilio" sort="Citterio, Attilio" uniqKey="Citterio A" first="Attilio" last="Citterio">Attilio Citterio</name>
</author>
<author><name sortKey="Cifuentes, Alejandro" sort="Cifuentes, Alejandro" uniqKey="Cifuentes A" first="Alejandro" last="Cifuentes">Alejandro Cifuentes</name>
</author>
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<author><name sortKey="Righetti, Pier Giorgio" sort="Righetti, Pier Giorgio" uniqKey="Righetti P" first="Pier Giorgio" last="Righetti">Pier Giorgio Righetti</name>
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<front><div type="abstract" xml:lang="en">For studying the Mr distribution and number of species in narrow‐range (2 pH‐unit wide, in the nominal pI 4–6 interval) carrier ampholytes from four commercial sources (Bio‐Lyte, Servalyt, Ampholine and Pharmalyte), a 2‐D technique was adopted consisting of a focusing step in a liquid phase (Rotofor, yielding 20 fractions) followed by orthogonal CE in both, acidic and basic buffers. As a final step, every other fraction was analyzed by CE‐MS. The findings: Ampholine contains 80 different Mr compounds, in the Mr interval 203 to 893 Da, for a total of 325 isoforms. Bio‐Lyte consists of 66 different Mr species, in the Mr range 388 to 835 Da, for a total of 436 isoforms. Servalyt is made of 199 different Mr compounds, in the Mr interval 204 to 907 Da, for a total of 1302 isoforms. Pharmalyte pH 4–6.5, comprises 217 amphoteres, in the Mr range 150 to 1179 Da, for a total of 812 isoforms. Pharmalyte appears to be the best brand, with the vast majority of species focusing sharply at their pI position and <5% “poor” species, distributed along the entire pH gradient, denoting an extremely shallow pH/mobility curve across the pI value.</div>
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<name sortKey="Citterio, Attilio" sort="Citterio, Attilio" uniqKey="Citterio A" first="Attilio" last="Citterio">Attilio Citterio</name>
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Mass distribution, polydispersity and focusing properties of carrier ampholytes for IEF II: pH 4–6 intervals

Mass distribution, polydispersity and focusing properties of carrier ampholytes for IEF II: pH 4–6 intervals

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri